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Gel filtration of water soluble protein of soybean and cotyledon of sprout at various growth stages by using Sephadex 6200 showed 5 fractions (A,B,C,D and E). According to gel filteration and disc gel electrophoresis, fraction B, C and D were identified as 11S,7S and 2S, respectively. Fraction A was turbid substance and fraction E showed 1 peak. During growth of sprout 7S decreased firstly, 2S secondly and 11S lastly in cotyledon. Fraction A increased until 6th day and decreased thereafter while E increased throughout the growth. In axis only two fractions (11S+7S and E) were showed, and 11S+7S fraction was little changed and fraction E increased slightly with the growth.
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